2025 CPT PLA Code Updates Are Live- How Labs Can Stay Ahead in U.S. Billing

2025 CPT PLA Code Updates Are Live: How Labs Can Stay Ahead in U.S. Billing

Effective October 1, 2025, the American Medical Association (AMA) introduced 25 new Proprietary Laboratory Analyses (PLA) codes, along with two deletions and one revision [1]. For U.S. labs and billing teams, these changes are more than just paperwork – they directly impact claims, revenue, and compliance.

With the right approach and tools like RevGen-i, labs can implement these updates efficiently, minimize errors, and safeguard revenue.

What Are PLA Codes and Why They Matter for Lab Billing

PLA codes represent specialized clinical lab tests that may be offered by a single laboratory or licensed to multiple providers. These codes cover advanced diagnostics such as oncology panels, transplant monitoring, and rare disease sequencing.

Accurate coding is crucial for U.S. labs and billing teams. Proper PLA code usage ensures claims are processed correctly, reduces denials, and helps capture full reimbursement for proprietary lab analyses.

PLA Code Updates: New, Revised, and Deleted Codes

The October 2025 AMA updates introduce several significant changes:

Deleted Codes: 0450U and 0451U, both related to multiple myeloma testing using LC-MS/MS.

Revised Code: 0333U now applies only to the HelioLiver™ Test by Helio Genomics® for liver oncology surveillance.

New Codes: 25 new PLA codes span oncology, transplant, neuro, infectious disease, and gastrointestinal diagnostics. Key additions include:

  • 0575U – HepatoTrack™ Liver RT-PCR 4 Genes, LuminoDx Inc
  • 0577U – GlycoKnow™ Ovarian, InterVenn Biosciences
  • 0585U – Labcorp® Plasma Complete™ cfDNA Panel
  • 0596U – Precivity-ApoE™, C2N Diagnostics

Labs should review the full list and ensure their U.S. lab billing services are updated to reflect these changes, verifying each code against internal testing menus and payer requirements.

Why Labs Should Act Fast on PLA Code Changes

PLA code updates are more than just administrative housekeeping – they directly impact revenue, compliance, and operational efficiency. Acting quickly ensures your lab billing services team stays ahead of potential issues.

Revenue Accuracy is critical. Submitting a claim with the wrong PLA code can lead to denials, delayed payments, or under-reimbursement.

Regulatory Compliance is another key factor. PLA codes are proprietary, and incorrect coding can trigger audits or compliance flags.

Workflow Efficiency matters too. Updating codes manually can be time-consuming and error-prone, slowing down your entire claims process.

Streamline Lab RCM with RevGen-i

Implementing PLA code updates doesn’t have to slow your lab down. RevGen-i, 3Gen Consulting’s AI-driven billing platform, acts as a smart co-pilot for U.S. lab billing services.

With RevGen-i, labs can:

  • Update new, revised, and deleted PLA codes across billing workflows
  • Validate test-to-code mapping to ensure claims are submitted accurately
  • Track potential denials and rework tied to proprietary lab tests
  • Generate real-time analytics to monitor revenue trends and reimbursement patterns

By integrating RevGen-i, labs can reduce manual errors, improve efficiency, and protect revenue – all while staying compliant with AMA coding requirements.

Takeaway: Stay Ahead in U.S. Lab Billing

The October 2025 PLA code updates are live, and labs that act proactively will benefit from smoother workflows, accurate claims, and optimized revenue. Leveraging smart tools like RevGen-i ensures that PLA code changes are implemented seamlessly, letting billing teams focus on efficiency rather than errors.

For labs looking to strengthen their lab billing services, accuracy alone isn’t enough. Proactive workflow management and AI-enabled automation are key to staying ahead in today’s evolving coding landscape.

Contact us to learn how RevGen-i can simplify PLA code updates for your lab.

 

References

[1] AMA, “CPT® Proprietary Laboratory Analyses (PLA) Codes: Long Descriptors,” 1 October 2025. Available: https://www.ama-assn.org/system/files/cpt-pla-codes-long.pdf.

The ABCs of Laboratory Billing- How Pathology Groups Can Stop Losing Revenue

The ABCs of Laboratory Billing: How Pathology Groups Can Stop Losing Revenue

Running a pathology group or diagnostic laboratory is challenging enough – but revenue cycle challenges make it even harder. If your lab claims are being denied due to missing prior authorizations, incorrect CPT codes, or “medical necessity not met” errors, you’re not alone. 

Laboratory billing is one of the most complex areas of U.S. healthcare revenue cycle management (RCM). With payers tightening scrutiny on molecular and genetic testing, expanding prior authorization requirements, and rolling

The Pathologist’s RCM Checklist Are Your Pathology Billing Systems Doing You Justice

The Pathologist’s RCM Checklist: Are Your Pathology Billing Systems Doing You Justice?

Your pathologists are diagnosing cancer. But your billing team? They’re diagnosing something else entirely: denials, underpayments, and revenue holes no one can seem to plug.

If that hits a little too close to home, you’re not alone.

We’ve worked with pathology labs across the U.S. – hospital-based, private groups, national reference labs – to know the symptoms: clean claims on paper but shrinking cash flow; pathology coding that looks fine until an audit hits; contract rates that haven’t changed in 5 years, but test complexity has doubled.

Before you hire another FTE or switch your pathology billing company again, run through this checklist. You’ll find out exactly where your pathology revenue cycle is bleeding – and how to fix it.

  1. Pathology Billing Services: Are You Getting Paid for Everything You Do?

Let’s be honest: pathology billing isn’t just billing. It’s a battlefield. 

If your medical billing partner isn’t a pathology billing company, they’re likely missing revenue opportunities you don’t even know exist.

Any of this sound familiar?

  • You’re billing Level 3s and 4s instead of 5s because it’s “safer”
  • Your team’s constantly cleaning up modifier messes (26, TC, 59, 91)
  • Reflex panels (IHC, FISH, NGS) aren’t billing downstream accurately
  • Tech/pro splits at hospital-based sites are inconsistent
  • You get clean claims… but collections are flatlining

3Gen Consulting’s pathology billing services use AI-powered claim scrubbing, modifier audits, test panel logic, and denial trend analysis to capture every reimbursable dollar while keeping you fully compliant.

  • Pathology Coding: Clean Claims Don’t Always Mean Correct

Let’s talk about the invisible revenue killer: pathology coding.

Pathology coding is one of the most overlooked revenue drains. Coders must go beyond basic CPT knowledge – they must understand stains, panels, molecular techniques, and payor nuances.

Ask yourself:

  • Are coders certified in pathology-specific coding?
  • Are they accurately coding for IHC, molecular, or genetic testing?
  • Is there QA in place to review coding before submission?
  • Is documentation of medical necessity being validated?

Even the cleanest billing process can’t save incomplete or inaccurate coding. If your team isn’t fluent in pathology coding nuance, you’re flying blind. With 3Gen’s team of certified pathology coders, clients achieve 95%+ accuracy, layered QA, audit-ready documentation, and real-time dashboards.

  • Payer Contracting: Are Your Rates Keeping Up With Test Complexity?

When’s the last time you renegotiated your payer contracts?

Most pathology groups haven’t touched their payer contracts in years. Meanwhile, payers tweak fee schedules, apply edits, and bundle services that should be billed separately.

Time to self-audit:

  • Have you renegotiated rates for molecular pathology?
  • Do your reimbursement rates align with MRF benchmark data?
  • Are just 2–3 poor-performing payers dragging down overall collections?
  • Have you tracked high-volume tests against peer lab payments?

Contracts are no longer “set and forget.” If you’re not renegotiating smarter and backing it up with data, you’re likely being underpaid for advanced pathology services. Our payer contracting team uses denial trends, test-level reimbursement data, and MRF benchmarking to arm you with hard facts to negotiate smarter.

  • Clinical Pathology Laboratories & Risk Adjustment: The New Revenue Frontier

Risk adjustment coding isn’t just for primary care. If your pathology lab serves Medicare Advantage or ACO populations, you’re sitting on untapped value. Pathology findings often uncover conditions that impact HCC coding and RAF scores – but most labs aren’t capturing them.

Here’s what to ask:

  • Are your coders flagging incidental findings like malignancies or chronic disease markers?
  • Do you have a system to track pathology-related HCC conditions?
  • Are your pathologists trained to document with risk adjustment in mind?

3Gen Consulting’s RiskGen-i platform integrates seamlessly with your LIS and EHR to identify, code, and track pathology-related risk conditions, aligning pathology with value-based care incentives.

  • Compliance, Audits & Denials: Are You Audit-Ready?

The scariest phrase in revenue cycle today: payer audit.

From CMS RADV audits to private payer clawbacks, pathology is under the microscope. And if your documentation, modifiers, or LCD coverage aren’t airtight, you’re exposed.

Sound familiar?

  • You’re still reacting to denials – not tracking patterns.
  • Appeals are a scramble, not a strategy.
  • You’re not sure if you’re compliant with No Surprises Act or new NCCI edits.
  • You haven’t done a proactive audit in over 6 months.

Compliance isn’t a checkbox. It’s a daily discipline – especially in pathology, where coding and documentation must be bulletproof. 3Gen Consulting supports labs with real-time denial analytics, audit response prep, and compliance documentation support, so you’re never caught off guard.

Final Diagnosis

It’s time to ask: Is your current pathology RCM partner keeping up – or holding you back? Most generic medical billing companies lack the clinical knowledge and test-level nuance needed to optimize pathology revenue. That’s where 3Gen Consulting steps in – with pathology-trained billers, coders, and contracting experts who understand your lab like it’s their own.

Want to see what your pathology revenue cycle is really doing behind the scenes? Let 3Gen Consulting run a free pathology medical billing health check. No fluff. No obligation. Just data-backed insight from a pathology billing company that knows your specialty. Book Your RCM Checkup Today.

Emerging U.S. Regulatory and Payer Trends in Laboratory Revenue Cycle Management image

Emerging U.S. Regulatory and Payer Trends in Laboratory Revenue Cycle Management

U.S. clinical labs face mounting regulatory challenges and shifting payer dynamics that are fundamentally changing how laboratory revenue cycle management (RCM) must be handled. Staying ahead means mastering compliance, optimizing payor contracting, and modernizing medical billing for laboratories to protect revenue and reduce expensive denials. If you manage a clinical lab, you’ve likely experienced how yesterday’s billing strategies no longer deliver. To survive – and thrive – you need to rethink your approach.

Regulatory Trends Shaping Laboratory Revenue Cycle Management

The regulatory spotlight on clinical laboratories in the U.S. is intensifying. The Centers for Medicare & Medicaid Services (CMS), the Food and Drug Administration (FDA), and new federal mandates have made lab RCM

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